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一步法人可溶性生長刺激表達(dá)基因2蛋白(sST2)酶聯(lián)免疫吸附檢測試劑盒

ELK008ES

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview

Product name:	EasyStep Human sST2(Soluble Suppression Of Tumorigenicity 2) ELISA Kit
Reactivity:	Human
Alternative Names:	s-ST2
Assay Type:	Sandwich
Sensitivity:	0.25 ng/mL
Standard:	30 ng/mL
Detection Range:	0.47-30 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	CD & Adhesion molecule
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sST2, and the Human sST2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human sST2. After TMB substrate solution is added, only those wells that contain Human sST2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sST2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
30.00	2.035	1.939
15.00	1.502	1.406
7.50	1.115	1.019
3.75	0.892	0.796
1.88	0.563	0.467
0.94	0.239	0.143
0.47	0.213	0.117
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant sST2 and the recovery rates were calculated by comparing the measured value to the expected amount of sST2 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-108%	101%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	82-97%	89%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sST2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.